Sunday, May 20, 2007

DNA detection


Picture taken from Hammond, D.M., Manetto, A., Gierlich, J., Azov, V.A., Gramlich, P.M.E., Burley, G.A., Maul, M., Carell, T. Angew. Chem. Int. Ed. 2007, 46, 4184. 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

In my mind, the future of cancer therapy is in the hands of personalized medicine; as the genetic makeup of each patient is different, it only makes sense to utilize customized therapies depending on the mutation affecting the individual. (Just look at the story of Iressa, and how well it works for Asian nonsmoking women with mutations in the EGFR tyrosine kinase domain). While the idea is a great one, personalized therapy is not a reality yet. Currently treatment options are too limited and often unspecific; methods to detect various disease markers are lacking. Personalized medicine depends upon the detection of minute amounts of DNA as well as isolation of particular genes from biological samples of interest, and the Carell group has developed a method to help with this.

Typically PCR is used for standard DNA amplification, although newer, more sophisticated techniques have allowed for detection of DNA down to the zeptomolar 10-21 level. (I'd never even heard of that one, my super-geeky husband had to fill me in). Unfortunately, such methods require the use of expensive/complex technology, so Carell and coworkers have developed a simple method of DNA detection based on standard black and white photography techniques. First, a pinacyanol dye was modified to contain an azide functionality, and several alkyne-containing oligodeoxyribonucleotides (ODNs) were synthesized. After a copper catalyzed Huisgen cycloaddition, various concentrations of modified ODNs were spotted down on commercial photopaper, irradiated, and developed according to known methods (standard photographic darkroom setup). This simple procedure allows for the detection of ~300 attomoles (10-18) of DNA!

17 comments:

Anonymous said...

Thank you, I have recently been searching for information about this topic for ages and yours is the best I have discovered so far.

Anonymous said...

Congratulations on having one of the most sophisticated blogs Ive come across in some time!  Its just incredible how much you can take away from something simply because of how visually beautiful it is.  Youve put together a great blog space --great graphics, videos, layout.  This is definitely a must-see blog!
Brussels Hotels

Reena said...

I greatly appreciate every one of the informative read here. I most certainly will spread the phrase about your site with people. Cheers.
Florence Hotels

puertas metalicas said...

Really useful information, thanks for the post.

www.sevilla-3d.com said...

I think everybody should glance at this.

sports betting software said...

really great place I highly recommend it

Anonymous said...

That′s a reasonablyDiablo III Gold number of years for just a in close proximity romance. My business is glad I've our lover here with me. May I actually ask you the reasons you can′t Cheap Guild Wars 2 Goldwork at the same location?

Anonymous said...

svzdrymwi www.louisvuittonhandbagsdiscountsale.com iriyaxvrb [url=http://www.louisvuittonhandbagsdiscountsale.com]louis vuitton handbags[/url] lpkaylhqj
waltzcwqe www.newdiscountlouisvuittonhandbag.com rickinomx [url=http://www.newdiscountlouisvuittonhandbag.com]louis vuitton handbags uk[/url] lifursrgx
tfzvdevla www.louisvuittononlineshoes.com exmxsmpuk [url=http://www.louisvuittononlineshoes.com]louis vuitton handbags on sale[/url] zpenbmgyv
uooshrqat www.louisvuittonluggageonline.com nazaoavii [url=http://www.louisvuittonluggageonline.com]louis vuitton wallet[/url] dpsrwhskb
tisergwns www.louisvuittonreplicbagsonline.com webtrgqcd [url=http://www.louisvuittonreplicbagsonline.com]louis vuitton discount[/url] pjmrjjujj

Anonymous said...

Wonderful article! This is the type of information that are supposed to be shared across the web.
Shame on the search engines for not positioning this publish higher!

Come on over and talk over with my web site . Thanks =)

Feel free to surf to my site - diet plans for women to lose weight fast

Anonymous said...

Wonderful article! This is the kind of information
that should be shared around the web. Shame on the search engines for not positioning this submit higher!
Come on over and seek advice from my site . Thanks
=)

Here is my web site - 時計コピー

Anonymous said...

This article is really a good one it helps new net users, who are
wishing in favor of blogging.

My webpage :: 激安プラダ バッグ

Anonymous said...

Have you ever considered about including a little bit more than just your articles?
I mean, what you say is important and everything. But think
about if you added some great photos or videos to give your posts more, "pop"!
Your content is excellent but with pics and videos, this blog could definitely
be one of the best in its niche. Awesome blog!


Look into my weblog: ロレックスコピー

Anonymous said...

I all the time emailed this blog post page to all my associates, since if like
to read it after that my links will too.

Here is my web page ... how to lose weight quickly

Anonymous said...

Attractive elеment of content. I just stumbled upοn your
web sitе and in accеѕsion capital
to say that I get аctually loved аcсount youг weblog posts.
Anyway I will be ѕubscribing to your feeԁs and even I fulfillment you get admisѕion tο constantly rapidlу.


Herе іs my homepаge: may quay phim

Anonymous said...

Тhe style for online games sliced kernel,
served apраrent. Do you Have got a Bambi fish poѕsible with οnly five casts.
Thаt Concluding Bullet traіn Rеcгeate, you Receive to buy
plοt clip from the publіshing сomρany.

3 The guy who but Own eхсellent abilitіеs Like poison, Bang, and team buffs to word form a sеriously synergetic team
of tοwerѕ. though it bгings fun and ferѵor, the intегiors оf your place or
backyard, you cаn Hоld the benefit to ρlay numerous types of games with only a few clіcκs.

Why is it Delight theѕe cluster of free online games.


Here is my рagе; game

Anonymous said...

After a stressful day your car will remind you that you should have Kurtis in your wardrobe.
The best benefit that you can wear the same things in
different ways and at different occasions. Most of these games
consist of designing outfits for dolls, styling fashion accessories or dressing up drop-dead
gorgeous models where you use mouse to click on the picture.
For instance, try tomato or soy sauce since they tend
to widen, rather than fashionable looks.

my web page: Ao so mi nam

qiongzhang55 said...

My partner and i on a regular basis e mailed this amazing site post site to all or any my associates,akatsuki costume given that if prefer to readit following my personal backlinks can way too.